Meyd873

  • A blog post?
  • A social media post?
  • A short story?
  • A product description?
  • Something else?

Tips:

  • The process can get messy, so cover your workspace with old sheets or plastic.
  • Experiment with different types of pulp, additives, and pressing techniques to create unique papers.
  • Making paper is an artisanal process, so don't be discouraged if your first attempts aren't perfect.

Materials Needed:

  • 1 cup of water
  • 1/2 cup of pulp (cotton linters or cotton rag)
  • A blender or food processor
  • A mixing bowl
  • A mold and deckle (you can purchase these or make your own)
  • A sponge
  • A pressing device (like a brayer, rolling pin, or heavy books)
  • Optional: additives like glitter, dyes, or textures (e.g., leaves, threads)

2.6. In‑Vitro Studies

  • Cell lines: MDA‑MB‑231 (TNBC) and NIH‑3T3 fibroblasts (ATCC).
  • Cytotoxicity: MTT assay after 72 h exposure to free DOX, DOX‑MEYD873 NPs, and blank NPs (0–20 µg mL⁻¹ DOX equivalent).
  • Cellular uptake: Confocal laser scanning microscopy (CLSM) after 4 h incubation with DOX‑labelled NPs (5 µg mL⁻¹).
  • Apoptosis: Annexin V/PI flow cytometry.
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